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Estimation of Flexible and Rigid Residues of Disulfide
Disulfide connectivity in peptides bearing at least two intramol. disulfide bonds is highly important for the structure and the biol. activity of the peptides. In that context, anal. strategies allowing a characterization of the cysteine pairing are of prime interest for chemists, biochemists, and biologists.
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Delivering quantum dot
Introduction. The unique spectral properties of luminescent semiconductor nanocrystals or quantum dots (QDs) have established them as attractive reagents for the long-term visualization of cellular structures and processes. 1–4 As a result, considerable effort has been invested in recent years in the development of facile yet robust methods for the specific cellular delivery of QDs, with a
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Modulation of Intracellular Quantum Dot to Fluorescent
The ligands used in this study included: (1) CL4, a small zwitterionic molecule bearing two carboxyl groups and one each secondary and tertiary amine moieties (length = ~1.8 nm) ; (2) DHLA-PEG 750-OME (PEG 750-OMe; length = ~6.8 nm) , a PEGylated ligand terminating with a neutral methoxy group; and (3) DHLA-PEG 600-NTA (PEG 600-NTA; length = ~6
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SCYL2 Protects CA3 Pyramidal Neurons from Excitotoxicity
Mice bearing the conditional allele of Bax were genotyped as described previously (Takeuchi et al., 2005). Generation of SCYL2 antibody. A peptide corresponding to the amino acids 24–39 (MGNPVTREFDVGRHIA [serum 7649]) of SCYL2 was synthesized by the Hartwell Center for Bioinformatics and Biotechnology and conjugated to glutaraldehyde
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Direct Evidence of an Elongation Factor
Conversely, tRNAs bearing aminoacyl moieties that contribute relatively little to the free energy of ternary complex formation bind strongly to EF-Tu (31, 36). Taken together with structural and kinetic evidence that EF-Tu undergoes large scale conformational changes during aa-tRNA binding and release ( 34 , 37 , – 39 ), this model posits a
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Estimation of Flexible and Rigid Residues of Disulfide
Disulfide connectivity in peptides bearing at least two intramol. disulfide bonds is highly important for the structure and the biol. activity of the peptides. In that context, anal. strategies allowing a characterization of the cysteine pairing are of prime interest for chemists, biochemists, and biologists.
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Super
Donkey-anti-mouse (Sigma, SAB3701101), donkey-anti-chicken (Invitrogen, SA172002) and donkey-anti-goat (Sigma, G6638) antibodies were conjugated with MARS dyes. Dyes . Most dyes are listed in
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Stress induces the expression of AtNADK
A potential candidate bearing a T-DNA insertion in the coding region of the AtNADK-1 gene was identified in the Syngenta Library (Sessions et al. 2002) and was named ndk1. The ndk1 mutant has a single T-DNA insertion in exon 9 (Fig. 6 a), as determined by the following segregation of the positive selection marker (BASTA).
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Gas
The reactant mixture was obtained by refluxing 120 g paraformaldehyde (prilled, Sigma) in 100 mL methanol (Honeywell Riedel-de-Haën) for 24 h. Subsequently, the solution was cooled to room temperature and filtered. A solution with a composition of 60 wt% FA, 38 wt% MeOH and 2 wt% H 2 O was obtained. 4.3 Analysis
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Estimation of Flexible and Rigid Residues of Disulfide
Disulfide connectivity in peptides bearing at least two intramol. disulfide bonds is highly important for the structure and the biol. activity of the peptides. In that context, anal. strategies allowing a characterization of the cysteine pairing are of prime interest for chemists, biochemists, and biologists.
-
Super
Donkey-anti-mouse (Sigma, SAB3701101), donkey-anti-chicken (Invitrogen, SA172002) and donkey-anti-goat (Sigma, G6638) antibodies were conjugated with MARS dyes. Dyes . Most dyes are listed in
